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Purpose
Collect and fix a standard list of tissues from the complete necropsy (see IMPC Gross Pathology & Tissue Collection SOP)
Trim the fixed tissues into cassettes for processing
Embed the tissues in paraffin in a standard orientation
Experimental Design
Procedure
- The following 29 tissues are collected and wet fixed at necropsy (according to IMPC Gross Pathology & Tissue Collection SOP)
Adrenal gland |
Heart |
Mammary gland |
Skin |
Thymus |
Brain |
Kidney |
Ovary |
Small intestine |
Thyroid |
Epididymis |
Large intestine |
Pancreas |
Spinal cord |
Trachea |
Esophagus |
Liver |
Prostate |
Spleen |
Urinary bladder |
Eye with optic nerve |
Lung |
Seminal vesicles |
Stomach |
Uterus |
Gall bladder |
Lymph node |
Skeletal muscle |
Testes |
- Trim the fixed tissue into labelled cassettes for processing according to Centre-specific SOP
- Process the cassettes according to Centre-specific SOP
- Embed the tissues in paraffin using the orientation below:
Tissue |
Number of Tissue Pieces |
Embedding Orientation |
Brain |
Up to 5 |
Transverse or Mid-sagittal |
Eye with optic nerve |
1 (per eye) |
Sagittal (optic nerve parallel to cassette surface) |
|
1 (per eye) |
Sagittal (within skull) |
Spinal cord |
Up to 6 |
Cross |
Thymus |
1 |
Flat |
Thyroid |
1 (with trachea) |
Cross |
Heart |
1 or 2 |
Cross or Sagittal |
Trachea |
1 (with thyroid) |
Cross |
Esophagus |
1 |
Cross |
Lung |
Up to 5 |
Cross or Sagittal |
Liver |
Up to 2 |
Cross |
Gall bladder |
1 (with liver) |
Cross |
Stomach |
Up to 2 |
Cross |
Small intestine |
1 |
Cross |
Large intestine |
1 |
Cross |
Pancreas |
1 |
Flat |
Spleen |
1 |
Flat or Cross |
Kidney |
1 or 2 |
Cross and Sagittal |
Adrenal gland |
At least 1 |
Cross or Sagittal |
Mammary gland |
At least 1 |
Flat |
Lymph node |
At least 1 |
Flat |
Skin |
Up to 5 |
Cross on edge |
Skeletal muscle |
At least 1 |
Cross |
Urinary bladder |
1 |
Flat |
Testes |
At least 1 |
Flat |
Epididymis |
At least 1 |
Flat |
Prostate |
1 |
Flat |
Seminal vesicles |
At least 1 |
Flat |
Ovary |
At least 1 |
Flat |
Uterus |
1 |
Flat or Cross |
Notes
The age of the animals at which they undergo this procedure is an important data. It is, however, derived from the date of birth and the date of necropsy of the animal, the latter being a metadata parameter. Similar situation applies to the number of animals that a centre has submitted to this procedure – it will also be derived and subject to quality control of the data.