To assess visible morphological defects in E18.5 placentas from lethal or subviable strains.
Experimental Design
Set up timed matings with heterozygous mice
Day 0 is defined as the midpoint of the prior dark cycle following the identification of a copulation plug.
Minimum number of animals : 1 mutant of any sex
Age at test: E18.5 and Older
Capture gross images (optional)
Collect tissue and genotype embryos.
Procedure
Set up timed mating with heterozygous animals. Dissect at a consistent time and collect >=2 placentas from homozygote embryos. Coordination with viability screen is at the centres discretion.
Assess placentas according to Gross Morphology parameters.
Generate gross images of placentas (optional) with scored defects and control placentas.
Collect tissue for genotyping
Process placentas for Histopathology, or other imaging (OPTIONAL - depending on center pipeline)
Scores will be shown per placenta and split by zygosity.
Notes
All genotypes should be collected using validated assays.
Y chromosome assay required for X-linked lethal strains.
Placentas may be processed for Histopathology or 3D Imaging