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Purpose
To detect mechanical/tactile sensitivity in the mouse paw
Experimental Design
Minimum number of mutant animals: 7 males + 7 females
Age at test: 16 weeks
Sexual dimorphism:
Procedure
- Baseline measurement
- Move the mice to the testing chambers and leave to acclimate for the specified length of time.
- Mice should be tested using the simplified up-down method (SUDO) of Bonin et al. (2014).
- When the mouse is still, apply the filament to the centre of the right hindpaw. Press the filament against the paw until it bends, then release.
- Mark the response '0' if the mouse does not react, or 'X' if it does react to the filament.
- Leave the mouse for a minimum of 2 minutes before presentation of the next filament.
- The next filament to be tested will depend on the response to the previous filament.
- If the mice did respond to the previous filament, they should be tested with the filament of the next smallest size. Once tested, record the response.
- If the mice did not respond to the previous filament, they should be tested with the filament of the next largest size. Once tested, record the response.
- Continue testing the mice until 5 trials have been completed.
- Challenge
- The challenge is administered after the baseline measurement has been completed.
- Test 1
- 24 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.
- Test 2
- 144 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.
Notes
This procedure is a pilot study from the Pain Phenotyping Pilot
A simplified up-down method (SUDO) for measuring mechanical nociception in rodents using von Frey filaments. Bonin RP, Bories C, De Koninck Y. Mol Pain. 2014 Apr 16;10:26. doi: 10.1186/1744-8069-10-26.PMID:24739328