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Purpose
To assess nocifensive behaviours triggered by the inflammatory response following a formalin injection.
Experimental Design
Minimum number of mutant animals: 5 males + 5 females
Age at test: 16 weeks
Age at test: 16 weeks
Procedure
1. Acclimatise mice in the testing room for at least 30 minutes before testing.
2. Anaesthetize mouse and administer formalin with a subcutaneous injection into the plantar surface of the chosen paw.
3. Place the mouse into the testing arena and start the recording. Run the recording for up to 90 minutes.
4. Remove the mouse from the arena and clean the arena.
5. Score the behaviour of the animals for nocifensive behaviors between 10 and 60 minutes post formalin administration.
2. Anaesthetize mouse and administer formalin with a subcutaneous injection into the plantar surface of the chosen paw.
3. Place the mouse into the testing arena and start the recording. Run the recording for up to 90 minutes.
4. Remove the mouse from the arena and clean the arena.
5. Score the behaviour of the animals for nocifensive behaviors between 10 and 60 minutes post formalin administration.
Notes
This procedure can be performed as a terminal or non-terminal procedure. If terminal, euthanize the mouse humanely after the recording.
This procedure is a pilot study from the Pain Phenotyping Pilot
This procedure is a pilot study from the Pain Phenotyping Pilot