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Purpose
To assess the fertility of homozygous knockout mice.
Experimental Design
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Minimum 1 hom x hom mating for fertile mice and minimum of 2 failed hom x hom matings attempts to proceed to secondary screen.
- Secondary screen: Minimum 2 Hom x 1 WT or Het (Male) , Minimum 2 Hom x 1 WT or Het (Female)
- Minimal age at test = minimal age at start of procedure = 8 weeks.
- Maximum age at test = To be confirmed, proposed 14 weeks
- Minimum length of test (time mice left in mating) 4 - 6 weeks
Procedure
- Homozygous mice (minimum age of 8 weeks, maximum age of 14 weeks) are mated for 4-6 weeks. Strains that produce no progeny or pregnant dams after 4 to 6 weeks progress to secondary screening.
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Secondary Male Infertility Screen:
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Set up 2 separate matings: male Hom x female WT or Het
- Observe matings for 4 to 6 weeks;
- Matings that result in visibly pregnant females (confirmed by dissection) or pups will be scored MALE FERTILE.
- Matings that do not result in pregnancy will be scored MALE INFERTILITY.
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Set up 2 separate matings: male Hom x female WT or Het
-
Secondary Female Infertility Screen
- Set up 2 separate matings: female Hom x male WT or Het
-
Observe matings for 4 to 6 weeks;
- Matings that result in visibly pregnant females (confirmed by dissection) or pups will be scored FEMALE FERTILE.
- Matings that do not result in pregnancy will be scored FEMALE INFERTILITY.
Notes
Pregnancies should be confirmed.
All annotations are based on yes/no scores.