IMPReSS Displaying Protocol Gross Morphology Placenta E14.5-E15.5

Gross Morphology Placenta E14.5-E15.5 IMPC_GPO_001

To assess visible morphological defects in E14.5-15.5 placentas from lethal strains.

Set up timed matings with heterozygous mice
Day 0 is defined as the midpoint of the prior dark cycle following the identification of a copulation plug.
Collect placentas at E14.5-E15.5 (at least 2 homozygotes scored)
1 WT stage matched control (if possible as mutant may be growth retarded) per litter
Capture gross images (optional)
Collect tissue and genotype embryos.

Set up timed mating with heterozygous animals. Dissect at a consistent time and collect >=2 placentas from homozygote embryos. Coordination with viability screen is at the centres discretion.
Assess placentas according to Gross Morphology parameters.
Generate gross images of placentas (optional) with scored defects and control placentas.
Collect tissue for genotyping
Process placentas for Histopathology, or other imaging (OPTIONAL - depending on center pipeline)
Scores will be shown per placenta and split by zygosity.

All genotypes should be collected using validated assays.

Y chromosome assay required for X-linked lethal strains.

Placentas may be processed for Histopathology or 3D Imaging

	Version	Type	Increment	Option	Data Type
Placenta Development IMPC_GPO_001_001	1.0	simpleParameter		normalabnormalunobservable	TEXT
Placenta Morphology IMPC_GPO_002_001	1.0	simpleParameter		normalabnormalunobservable	TEXT
Placenta Vasculature IMPC_GPO_003_001	1.0	simpleParameter		normalabnormalunobservable	TEXT
Placenta Size IMPC_GPO_004_001	1.0	simpleParameter		normalabnormalunobservable	TEXT
Umbilical cord morphology IMPC_GPO_005_001	1.0	simpleParameter		normalabnormalunobservable	TEXT
Comment on image IMPC_GPO_006_001	1.0	simpleParameter			TEXT
Images IMPC_GPO_007_001	1.0	seriesMediaParameter	Minimum: 1		IMAGE
Pale placenta IMPC_GPO_018_001	1.0	simpleParameter		noyesunobservable	TEXT

	Version	Type	Data Type
Experimenter ID IMPC_GPO_008_001	1.0	procedureMetadata	TEXT
Equipment ID IMPC_GPO_009_001	1.0	procedureMetadata	TEXT
Equipment Manufacturer IMPC_GPO_010_001	1.0	procedureMetadata	TEXT
Equipment Model IMPC_GPO_011_001	1.0	procedureMetadata	TEXT
Fixative IMPC_GPO_012_001	1.0	procedureMetadata	TEXT
Time of Dissection IMPC_GPO_013_001	1.0	procedureMetadata	TIME
Somite Stage IMPC_GPO_014_001	1.1	procedureMetadata	INT
Time of dark cycle start IMPC_GPO_015_001	1.0	procedureMetadata	TIME
Time of dark cycle end IMPC_GPO_016_001	1.0	procedureMetadata	TIME
Date equipment last calibrated IMPC_GPO_017_001	1.1	procedureMetadata	DATE