Construct and analyse digital X-ray images in immobilised mice using a Faxitron X-Ray system or NTB digital X-ray scanner.
- Minimum number of animals : 4M + 4F
- Age at test: Week 14
Perform steps 3.1 and 3.2 unless the mouse is coming from DEXA analysis
3.1 Anesthetize the mice.
3.2 Monitor the animal carefully until unconsciousness by ensuring the mouse is adequately sedated.
3.3 Mount the anesthetized mouse onto an X-ray permeable plate. Curve the tail along the left side of the mouse. (Note: this will enable identification of the X-ray as a dorsal or ventral image.)
Faxitron X-ray system
3.3.1 Insert the plate into the Faxitron X-ray system.
3.3.2 Take an image using the software that is supplied by the X-ray scanner.
3.3.3 Set the machine to a voltage of 25kV with an integration time of 40ms.
3.3.4 Take an image using the iX-Pect software that is supplied by the X-ray scanner.
NTB digital X-ray scanner
3.4 Remove the mouse once the image is captured. Place the mouse on a heated mat, set at 37°C, in a cage and monitor closely until consciousness is fully regained.
3.5 Analyze the image.
3.6 Or continue with ABR.
The protocol described herein employs anaesthesia using intraperitoneal (IP) administration; however, anaesthesia using isoflurane is also suitable.
Digit integrity IMPC_XRY_031_001 Definition: The state of being whole and undivided.