Listing All Pipelines

IMPC Pipeline IMPC_001

The IMPC Pipeline is a core set of Procedures and Parameters to be collected by all phenotyping centers.

Harwell HRWL_001

Harwell extra parameters

TCP Pipeline TCP_001

Toronto Centre for Phenogenomics's (TCP) pipeline for extra parameters and procedures

ICS ICS_001

ICS's pipeline for extra procedures and parameters

JAX Pipeline JAX_001

UCD Pipeline UCD_001

German Mouse Clinic HMGU_001

German Mouse Clinic Pipeline for IMPC Project _ Rotarod as an additional center-specific Procedure

MGP Select Pipeline MGP_001

BCM Pipeline BCM_001

CCP Pipeline CCP_001

Czech Centre for Phenogenomics (CCP) Pipeline

TCP Interval Pipeline TCPIP_001

UCD Interval Pipeline UCDIP_001

Salmonella Challenge SLM_001

Mutant mice and wild-type controls (8/group) have blood collected from the tail (50 ul) at 6 weeks of age for peripheral blood leukocyte and haematology analysis. One week later mice are infected with Salmonella Typhimurium M525 strain containing TETc via the intravenous route. Weights are recorded daily and any mice that have lost greater than 20% or that exhibit clinical signs are sacrificed. At day 14-post infection half the mutants and wild type controls are sacrificed with spleen, liver and caecal contents collected. Bacterial counts in the organs are determined by serial plating and portion of the spleen and liver are formalin fixed and paraffin embedded. At day 28-post infection the remaining mutants are sacrificed and processed as above. In addition blood is collected by cardiac puncture and anti-TETc antibodies in the serum are determined by ELISA for total IgG, IgG1 and IgG2a.

Trichuris challenge TRC_001

Mutant mice and wild-type controls (6 females/group) are infected with Trichuris muris eggs by oral gavage. Weights are recorded daily and any mice that have lost greater than 20% or that exhibit clinical signs are culled. At day 33 post challenge the mice are sacrificed with the caecum collected and assessed for the presence or absence of worms. In addition blood is collected by cardiac puncture and serum isolated for confirmation of productive infection.

DSS challenge DSS_001

Mutant mice and wild-type controls (2 female and 2 males/group) receive 1.5% dextran sodium sulphate (DSS) in drinking water for 7 days. Weights are recorded daily and any mice that have lost greater than 20% or that exhibit clinical signs are sacrificed. At day 10 mice are sacrificed and samples from the medial and distal gut are collected for histological analysis. A pathology scoring system is used to assess the effect of the DSS in a blinded manner.

Riken late adult RBRCLA_001

Pipeline for Riken's late adult procedures

Harwell late adult HRWLLA_001

Harwell late adult

Jax late adult JAXLA_001

Jax late adult

UCD late adult UCDLA_001

UCD late adult

TCP late adult TCPLA_001

TCP late adult

ICS late adult ICSLA_001

ICS late adult

BCM late adult BCMLA_001

Baylor late adult

KMPC late adult KMPCLA_001

KMPC late adult

Nanjing late adult NINGLA_001

Nanjing late adult

Harwell interval pipeline HRWLIP_001

Pipeline for Harwell procedures between the early adult and late adult pipelines

JAX interval pipeline JAXIP_001

Pipeline for procedures between the early adult and late adult pipelines

CCP Interval Pipeline CCPIP_001

CCP Interval Pipeline; phenotyping between the Early Adult (EA) and Late Adult (LA) pipelines.

CCP Late Adult CCPLA_001

CCP Late Adult pipeline

GMC late adult HMGULA_001

Pipeline for GMC's late adult procedures

Harwell Ageing Screen HAS_001

Legacy Pipelines

EUMODIC Pipeline 1 ESLIM_001

EUMODIC Pipeline 1

EUMODIC Pipeline 2 ESLIM_002

EUMODIC Pipeline 2

GMC Pipeline GMC_001

GMC Pipeline

MGP Pipeline M-G-P_001

MGP Pipeline

EUMODIC Pipeline 3 ESLIM_003

EUMODIC Pipeline 3